Light sheet microscopy of blood vessels in mouse brain in vivo I. V. Fedosov, O. V. Semyachkina-Glushkovskaya, V. V. Tuchin
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ArticleContent type: Текст Media type: электронный Subject(s): световая микроскопия | Эванса синий | кровообращение | мозгGenre/Form: статьи в журналах Online resources: Click here to access online
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Proceedings of SPIE Vol. 11067 : Saratov fall meeting 2018 : Computations and data analysis: from nanoscale tools to brain functions. P. 1106711-1-1106711-6Abstract: A method for intravital light sheet microscopy of blood vessels of mouse brain cortex is proposed. The use of tilted microscope lens mount and long working distance of microscope lens ensure high contrast images in both scattered light and fluorescence modes without immersion. Fluctuations of laser speckles related with the blood flow has been observed in scattered mode. In fluorescence mode distribution of Evans Blue dye over blood vessel cross-section was visualized.
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A method for intravital light sheet microscopy of blood vessels of mouse brain cortex is proposed. The use of tilted microscope lens mount and long working distance of microscope lens ensure high contrast images in both scattered light and fluorescence modes without immersion. Fluctuations of laser speckles related with the blood flow has been observed in scattered mode. In fluorescence mode distribution of Evans Blue dye over blood vessel cross-section was visualized.
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