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Библиогр.: 25 назв.

Skin Carcinoma is one of the most frequent and spreaded type of skin cancers. Its diagnosis and the resulting surgical treatment are complicated due to the lack of precise surgical margin delineation approaches. Optical methods are very promising and effective tools for bringing a clinically compatible solution to this problem because of their non-invasive principles and high informativity. Spatially Resolved Multi-Modal Spectroscopy (SRMMS) provide in vivo information with depth resolution about cancer specific features with high precision by analyzing skin Diffuse Reflectance and and Autofluorescence spectra at specific locations on tissue. Due to multiple light scattering, absorption and reflectance of the photons in the biological tissue it results in very poor photons penetration into the deeper areas and, consecutively, low depth sensitivity. Potential solution of this problem is optical clearing technology. In this work, we analyzed the time kinetics of DR and multiply excited AF spectra collected on an ex vivo skin strip on top of ex vivo skin/gel hybrid model following topical application of OCA at 4 different acquisition distances and 5 different excitation wavelengths. We then investigated the possible impact of probe pressure as well as drying of the skin sample on the spectroscopic signals, besides the optical clearing effect. The results obtained showed that the studied OCA solutions reduced autofluorescence of the skin and improved the depth sensitivity of the spectroscopy applied to the skin. Another notable effect is the strong increasing of collected exogenous fluorescence of a bottom layer in a “dry” conditions.