Thidiazuron-induced formation of strawberry microshoots on different nutrient media E. V. Ambros, S. Y. Toluzakova, D. Komarova, T. I. Novikova
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ArticleContent type: Текст Media type: электронный Subject(s): земляника | микропобеги | питательные среды | тидиазуронGenre/Form: статьи в журналах Online resources: Click here to access online
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AIP Conference Proceedings Vol. 2390. P. 030002-1-030002-5Abstract: The present study was aimed to improve in vitro shoot proliferation of octoploid strawberry cultivar ‘Solnechnaya polyanka’ on different nutrient media (MS, B5 or MS+B5), supplemented with various concentrations (2.0, 4.0, 8.0 ȝM) of thidiazuron (TDZ). The best regeneration frequency (100%) with the highest rate of strawberry axillary shoot proliferation (14.1 ± 1.24 shoots per explant) and maximum shoot length (1.92 ± 0.04 cm) were achieved using precultivation of explants for 3 days on induction media B5 supplemented with 4.0 ȝM TDZ followed by cultivation of regenerants on hormone-free media B5 for 6 weeks.
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The present study was aimed to improve in vitro shoot proliferation of octoploid strawberry cultivar ‘Solnechnaya polyanka’ on different nutrient media (MS, B5 or MS+B5), supplemented with various concentrations (2.0, 4.0, 8.0 ȝM) of thidiazuron (TDZ). The best regeneration frequency (100%) with the highest rate of strawberry axillary shoot proliferation (14.1 ± 1.24 shoots per explant) and maximum shoot length (1.92 ± 0.04 cm) were achieved using precultivation of explants for 3 days on induction media B5 supplemented with 4.0 ȝM TDZ followed by cultivation of regenerants on hormone-free media B5 for 6 weeks.
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