Development of DNA aptamers to native EpCAM for isolation of lung circulating tumor cells from human blood G. S. Zamay, O. S. Kolovskaya, T. I. Ivanchenko [et al.]
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ArticleSubject(s): ДНК-аптамеры | систематическая эволюция лигандов экспоненциальным обогащением | немелкоклеточный рак легкого | молекула клеточной адгезии эпителия | циркулирующие опухолевые клетки | конфокальная микроскопия | кровьGenre/Form: статьи в журналах Online resources: Click here to access online
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Cancers Vol. 11, № 3. P. 351 (1-14)Abstract: We selected DNA aptamers to the epithelial cell adhesion molecule (EpCAM) expressed on primary lung cancer cells isolated from the tumors of patients with non-small cell lung cancer using competitive displacement of aptamers from EpCAM by a corresponding antibody. The resulting aptamers clones showed good nanomolar affinity to EpCAM-positive lung cancer cells. Confocal microscopy imaging and spectral profiling of lung cancer tissues confirmed the same protein target for the aptamers and anti-EpCAM antibodies. Furthermore, the resulted aptamers were successfully applied for isolation and detection of circulating tumor cells in clinical samples of peripheral blood of lung cancer patients.
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We selected DNA aptamers to the epithelial cell adhesion molecule (EpCAM) expressed on primary lung cancer cells isolated from the tumors of patients with non-small cell lung cancer using competitive displacement of aptamers from EpCAM by a corresponding antibody. The resulting aptamers clones showed good nanomolar affinity to EpCAM-positive lung cancer cells. Confocal microscopy imaging and spectral profiling of lung cancer tissues confirmed the same protein target for the aptamers and anti-EpCAM antibodies. Furthermore, the resulted aptamers were successfully applied for isolation and detection of circulating tumor cells in clinical samples of peripheral blood of lung cancer patients.
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