Multibiomarker approach to fipronil exposure in the fish Dicentrarchus labrax under two temperature regimes S. Dallarés, P. Dourado, I. Sanahuja [et al.]
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ArticleContent type: Текст Media type: электронный Subject(s): фипронил | морские окуни | биомаркеры | окислительный стресс | изменение климатаGenre/Form: статьи в журналах Online resources: Click here to access online
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Aquatic toxicology Vol. 219. P. 105378 (1-12)Abstract: Fipronil is a phenylpyrazole insecticide widely used to control pests in agriculture even though evidence of
harmful side effects in non-target species has been reported. A comprehensive study on the effects of dietary
administration of Regent®800WG (80 % fipronil) in European sea bass juveniles was carried out under two
temperature regimes: a) natural conditions, and b) 3 °C above the natural temperature (an increase predicted for
the NW Mediterranean by the end of this century). Fipronil was added to the fish food (10 mg fipronil /Kg feed)
and the effects were studied at several time points including right before administration, 7 and 14 days after
daily fipronil feed and one-week after the insecticide withdrawal from the diet (depuration period). A wide array
of physiological and metabolic biomarkers including feeding rate, general condition indices, plasma and epidermal
mucus metabolites, immune response, osmoregulation, detoxification and oxidative-stress markers and
digestive enzymes were assessed. General linear models and principal component analyses indicated that regardless
of water temperature, fipronil resulted in a significant alteration of several of the above listed biomarkers.
Among them, glucose and lactate levels increased in plasma and decreased in epidermal mucus as
indicators of a stress response. Similarly, a depletion in catalase activity and higher lipid peroxidation in liver of
fipronil-exposed fish were also indicative of an oxidative-stress condition. Fipronil induced a time dependent
inhibition of Cytochrome P450-related activities and an increase of phase II glutathione-S-transferase. Moreover,
fipronil administration was able to reduce the hypo-osmoregulatory capability as shown by the increase of
plasmatic osmolality and altered several digestive enzymes including trypsin, lipase, alpha amylase and maltase.
Finally, analyses in bile and muscle confirmed the rapid clearance of fipronil but the persistence of the metabolite
fipronil-sulfone in bile even after the 7-day depuration period. Altogether, the results reveal a notable
impact of this compound on the physiological condition of the European sea bass. The results should be considered
in future environmental risk assessment studies since fipronil could be hazardous to fish species, particularly
those inhabiting estuarine ecosystems exposed to the discharge of agriculture runoffs where this pesticide
is mainly used.
Библиогр.: с. 105378 (10-12)
Fipronil is a phenylpyrazole insecticide widely used to control pests in agriculture even though evidence of
harmful side effects in non-target species has been reported. A comprehensive study on the effects of dietary
administration of Regent®800WG (80 % fipronil) in European sea bass juveniles was carried out under two
temperature regimes: a) natural conditions, and b) 3 °C above the natural temperature (an increase predicted for
the NW Mediterranean by the end of this century). Fipronil was added to the fish food (10 mg fipronil /Kg feed)
and the effects were studied at several time points including right before administration, 7 and 14 days after
daily fipronil feed and one-week after the insecticide withdrawal from the diet (depuration period). A wide array
of physiological and metabolic biomarkers including feeding rate, general condition indices, plasma and epidermal
mucus metabolites, immune response, osmoregulation, detoxification and oxidative-stress markers and
digestive enzymes were assessed. General linear models and principal component analyses indicated that regardless
of water temperature, fipronil resulted in a significant alteration of several of the above listed biomarkers.
Among them, glucose and lactate levels increased in plasma and decreased in epidermal mucus as
indicators of a stress response. Similarly, a depletion in catalase activity and higher lipid peroxidation in liver of
fipronil-exposed fish were also indicative of an oxidative-stress condition. Fipronil induced a time dependent
inhibition of Cytochrome P450-related activities and an increase of phase II glutathione-S-transferase. Moreover,
fipronil administration was able to reduce the hypo-osmoregulatory capability as shown by the increase of
plasmatic osmolality and altered several digestive enzymes including trypsin, lipase, alpha amylase and maltase.
Finally, analyses in bile and muscle confirmed the rapid clearance of fipronil but the persistence of the metabolite
fipronil-sulfone in bile even after the 7-day depuration period. Altogether, the results reveal a notable
impact of this compound on the physiological condition of the European sea bass. The results should be considered
in future environmental risk assessment studies since fipronil could be hazardous to fish species, particularly
those inhabiting estuarine ecosystems exposed to the discharge of agriculture runoffs where this pesticide
is mainly used.
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