TY  - SER
AU  - Frank,Yuliya A.
AU  - Mardanov,Andrey V.
AU  - Beletsky,Alexey V.
AU  - Ivasenko,Denis A.
AU  - Pimenov,Nikolay V.
AU  - Karnachuk,Olga V.
AU  - Ravin,Nikolay V.
AU  - Kadnikov,Vitaly V.
TI  - Variability of the composition of the microbial community of the deep subsurface thermal aquifer in Western Siberia
KW  - микробное сообщество
KW  - молекулярный анализ
KW  - метаногены
KW  - подземная биосфера
KW  - термальные воды
KW  - Западная Сибирь
KW  - статьи в журналах
N1  - Библиогр.: с. 771-772
N2  - The deep subsurface biosphere is one of the least studied ecosystems on Earth, containing communities of extremophilic microorganisms. The present work was aimed at molecular genetic characterization of microbial communities of underground thermal waters in Western Siberia, lying at depths of 2–3 km. Water samples were collected from the 5P oil-exploration well, drilled to a depth of 2.8 km near the village Chazhemto (Tomsk region). The water had a temperature of about 20°C, a neutral pH and a low redox potential (–304 mV). Underground aquifers have a complex structure and may contain both planktonic microorganisms and those immobilized on the surface of rocks in the form of biofilms, which may be washed out and detected in the water flowing out of the well. Community composition was analyzed by amplification and pyrosequencing of the 16S rRNA gene fragments in seven water samples taken at different times during 26 hours. Bacteria, which constituted about half of the community, were represented mainly by uncultured lineages of the phyla Firmicutes, Ignavibacteria, Chloroflexi, Bacteroidetes, and Proteobacteria. Archaea belonged mainly to known methanogens of the genera Methanothermobacter, Methanosaeta, and Methanomassiliicoccus. Analysis of the samples taken at different times revealed large variations in the content of most groups of bacteria, with a decrease in Firmicutes abundance accompanied by an increase in the shares of Ignavibacteria and Chloroflexi. The share of archaea of the genus Methanothermobacter varied slightly during the day, while significant variations were observed for the phylotypes assigned to Methanosaeta and Methanomassiliicoccus. Hydrogenotrophic archaea of the genus Methanothermobacter are probably a permanent component of the microbial community occurring in the planktonic state, while most of the identified groups of bacteria are present in biofilms or spatially localized parts of the underground water reservoir, the material of which accidentally enters the well
UR  - http://vital.lib.tsu.ru/vital/access/manager/Repository/vtls:000636524
ER  -