E-catalog        

Библиогр.: 3 назв.

The regenerative process mechanisms of the nervous system after brain ischemia remain unclear and request new research approach for detailed and reliable observation. In the present study recent noninvasive technique, fast macromolecular proton fraction (MPF) mapping, was compared with immunohistochemistry to assess recovery of the brain regions that were damaged by ischemia in rat focal ischemia model (MCAO). The present study revealed regions with dramatic decrease of MPF intensity in the striatum of rats after MCAO from the first day after ischemia to the fifty-sixth day (the last timepoint). Immunohistochemical analysis of this regions have shown neuronal and myelin loss, but the number of neuronal and glial progenitors increased in comparison with control group. Comparison of MPF maps and micrographs have revealed exact correspondence between the border of glial scar, which limits the place of nerve cell death, and regions of active neuronal progenitors migration with the regions of hypointensity on the MPF maps. The results provide histolocical validation of MPF as a noninvasive technique for monitoring of regenerative processes in the regions of neuronal and myelin loss after ischemia.