Diffusion of methylene blue in human dentin in the presence of glucose: in vitro study A. A. Selifonov, V. V. Tuchin
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ArticleContent type: Текст Media type: электронный Subject(s): метиленовый синий | глюкоза | дентин | стоматология | диффузияGenre/Form: статьи в журналах Online resources: Click here to access online
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Proceedings of SPIE Vol. 11065 : Saratov fall meeting 2018 : Optical and nano-technologies for biology and medicine. P. 110651Y-1-110651Y-9Abstract: The work is devoted to the determination of the diffusion coefficients of methylene blue (MB) in pure aqueous and 40%-glucose-aqueous solutions in human tooth dentinal sections in vitro using diffuse reflectance optical spectroscopy and the free diffusion model. The determination of the diffusion coefficient of MB in a biological tissue was based on the measurement of the temporal kinetics of changes in the diffuse reflectance spectrum. Obviously, the diffusion rate along the dentinal tubules should be the greatest, close to that in water, taking into account some reduction in speed due to interaction with the walls, since they are filled up with a liquid with a high water content. In addition, the density of the dentinal tubules is large enough, so we can expect that their contribution to the total diffusion will be significant. It was determined that the diffusion coefficient, for 0.001% MB in an aqueous solution is (6.74±1.32)·10-6 cm2 and in 40%-glucose solution is (3.89±0.85)·10-6 cm2/s
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The work is devoted to the determination of the diffusion coefficients of methylene blue (MB) in pure aqueous and 40%-glucose-aqueous solutions in human tooth dentinal sections in vitro using diffuse reflectance optical spectroscopy and the free diffusion model. The determination of the diffusion coefficient of MB in a biological tissue was based on the measurement of the temporal kinetics of changes in the diffuse reflectance spectrum. Obviously, the diffusion rate along the dentinal tubules should be the greatest, close to that in water, taking into account some reduction in speed due to interaction with the walls, since they are filled up with a liquid with a high water content. In addition, the density of the dentinal tubules is large enough, so we can expect that their contribution to the total diffusion will be significant. It was determined that the diffusion coefficient, for 0.001% MB in an aqueous solution is (6.74±1.32)·10-6 cm2 and in 40%-glucose solution is (3.89±0.85)·10-6 cm2/s
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