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Библиогр.: 3 назв.

In the present study, we performed the in vitro study of the impact of cisplatin, well known chemotherapeutic agent, on tumor associated macrophages (TAMs). We used model system of TAMs which was obtained by the stimulation of primary monocytes with supernatants of cancer cell MCF7 (breast adenocarcinoma) and Colo206F (colorectal cancer). Cisplatin and TAMs interaction was examined through the full-transcriptome next-generation sequencing (NGS) of TAMs before and after cisplatin treatment within two different models. After sequencing, following bioinformatic analysis of obtained data was performed. Obtained results demonstrated, that TAMs after cisplatin treatment activate numerous pathways involved in tumor progression and immune response. Data analysis revealed, that TAMs in Colo206F microenvironment acquire mostly anti-tumor program while in the present of MCF7 supernatant — tumor-supporting program. This opens the prospects for the search for new markers of tumor response to chemotherapeutic drugs and immunomodulatory approaches to increase the effectiveness of chemotherapy.