Analysis of the metaphase chromosome karyotypes in imaginal discs of Aedes communis, Ae. punctor, Ae. intrudens, and Ae. rossicus (Diptera: Culicidae) mosquitoes S. S. Alekseeva, Y. V. Andreeva, I. E. Wasserlauf [et al.]
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ArticleContent type: Текст Media type: электронный Subject(s): комары | митотические хромосомы | имагинальные дискиGenre/Form: статьи в журналах Online resources: Click here to access online
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Insects Vol. 11, № 1. P. 63 (1-9)Abstract: In this study, cytogenetic analysis of the metaphase chromosomes from imaginal
discs of Aedes (Diptera: Culicidae) mosquitoes—Aedes communis, Ae. punctor, Ae. intrudens, and
Ae. rossicus—was performed. The patterns of C-banding and DAPI staining of the heteroñhromatin
and the length of the chromosomes demonstrate species specificity. In particular, the Ae. punctor
chromosomes are the shortest compared with Ae. communis, Ae. intrudens, and Ae. rossicus, and they
also carry additional C and DAPI bands in intercalary regions. The Ae. intrudens chromosomes are
the longest, they have pericentromeric C bands, and they almost lack any DAPI bands near the
centromere of chromosome 3 versus Ae. communis, which has the largest pericentromeric DAPI blocks
in all three chromosome pairs. Ae. rossicus also possesses DAPI bands in the centromeric regions of
all chromosomes, but their staining is weaker compared with those of Ae. communis. Therefore, the
analysis of karyotypes is a tool for species-level identification of these mosquitoes.
Библиогр.: 36 назв.
In this study, cytogenetic analysis of the metaphase chromosomes from imaginal
discs of Aedes (Diptera: Culicidae) mosquitoes—Aedes communis, Ae. punctor, Ae. intrudens, and
Ae. rossicus—was performed. The patterns of C-banding and DAPI staining of the heteroñhromatin
and the length of the chromosomes demonstrate species specificity. In particular, the Ae. punctor
chromosomes are the shortest compared with Ae. communis, Ae. intrudens, and Ae. rossicus, and they
also carry additional C and DAPI bands in intercalary regions. The Ae. intrudens chromosomes are
the longest, they have pericentromeric C bands, and they almost lack any DAPI bands near the
centromere of chromosome 3 versus Ae. communis, which has the largest pericentromeric DAPI blocks
in all three chromosome pairs. Ae. rossicus also possesses DAPI bands in the centromeric regions of
all chromosomes, but their staining is weaker compared with those of Ae. communis. Therefore, the
analysis of karyotypes is a tool for species-level identification of these mosquitoes.
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