Влияние химических ингибиторов генов стволовости на способность дифференцированных клеток линий рака молочной железы к индукции дедифференцировки до стволовых клеток с образованием маммосфер Д. С. Долгашева, М. К. Ибрагимова, М. М. Цыганов
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ArticleContent type: Текст Media type: электронный Other title: The effect of chemical inhibitors of the stemness genes on the ability of differentiated breast cancer cell lines to induce dedifferentiation to tumor stem cells with formation of mammospheres [Parallel title]Subject(s): дифференцированные опухолевые клетки | опухолевые стволовые клетки | маммосферы | рак молочной железы | экспериментальные исследованияGenre/Form: статьи в сборниках Online resources: Click here to access online
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Перспективы развития фундаментальных наук. Т. 4 : сборник научных трудов XIX Международной конференции студентов, аспирантов и молодых ученых, 26-29 апреля 2022 г Т. 4 : Биология и фундаментальная медицина. С. 26-28Abstract: In the present study, we studied the ability of differentiated tumor cells to stem plasticity and the formation of mammospheres in the presence of stemness genes inhibitors. We used breast cancer cell lines: BT- 549, MDA-MB-231, MCF-7, SK-BR-3 and T47D. The cells were cultured in complete nutrient media RPMI- 1640, IMDM, and DMEM. The genetic landscape of CNA was examined on CytoScan HD Array microarrays, and cellular transcriptome assessment on Clarium S Assay platform. The subpopulation composition of cell cultures was studied by flow cytometry. Negative sorting of differentiated cells was performed on a Sony SH800 device. All cell cultures, except for BT-549, were shown to contain two or more amplifications of stemness genes. MCF-7 and T47D cells were selected for experiments based on the results of subpopulation composition analysis. Wild-type MCF-7 cells were shown to spontaneously form mammospheres without IL6 induction on day 14 of cultivation. Differentiated MCF-7 cells form complete mammospheres on day 14 only in the presence of IL6. The experiment using stemness gene inhibitors was performed on T47D cells. T47D were found to be capable of spontaneous dedifferentiation and mammosphere formation. Chemical inhibitors in combination were shown to effectively block the dedifferentiation of T47D tumor stem cells.
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In the present study, we studied the ability of differentiated tumor cells to stem plasticity and the formation of mammospheres in the presence of stemness genes inhibitors. We used breast cancer cell lines: BT- 549, MDA-MB-231, MCF-7, SK-BR-3 and T47D. The cells were cultured in complete nutrient media RPMI- 1640, IMDM, and DMEM. The genetic landscape of CNA was examined on CytoScan HD Array microarrays, and cellular transcriptome assessment on Clarium S Assay platform. The subpopulation composition of cell cultures was studied by flow cytometry. Negative sorting of differentiated cells was performed on a Sony SH800 device. All cell cultures, except for BT-549, were shown to contain two or more amplifications of stemness genes. MCF-7 and T47D cells were selected for experiments based on the results of subpopulation composition analysis. Wild-type MCF-7 cells were shown to spontaneously form mammospheres without IL6 induction on day 14 of cultivation. Differentiated MCF-7 cells form complete mammospheres on day 14 only in the presence of IL6. The experiment using stemness gene inhibitors was performed on T47D cells. T47D were found to be capable of spontaneous dedifferentiation and mammosphere formation. Chemical inhibitors in combination were shown to effectively block the dedifferentiation of T47D tumor stem cells.
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